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KMID : 0361019950380081201
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Korean Journal of Otolaryngology - Head and Neck Surgery
1995 Volume.38 No. 8 p.1201 ~ p.1205
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Differentiation of Human Nasal Epithelial Cells(HNEC)
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Yoon Joo-Heon
Lee Jeung-Gweon
Choe Jin-Woo
Park In-Yong
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Abstract
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The aim of this paper is to investigate whether human nasal epithelial cells can differentiate into ciliated cells and secretory cells simultaneously by the floating method. Dissociated human nasal epithelial cells from nasal polyp were cultured in Ham’s F12-Dulbecco’s mochified Eagle medium 1/1 Supplemented with 10% NU serum, choleratoxin, retinoic acid and antibiotics. We observed the morphologic changes after floating by the transmission electron microscope. Electron lucent cornified cells in upper layer and flat cells in basal layer were noted at the time of confluence. Two to three layers of the cuboidal cells which mean some differentiation of squamous-like cells were observed 4 days after floating. Seven days after floating, the progression of cell differentiation by observing more number of cilia produced along with ciliated epithelial cells was noted. Electron dense secretory granules were located near the nucleus and electron lucent secretory granules were located near the secretory cell membrane. Fourteen days after floating, completely differentiated ciliated epithelial cells and the cells which has electron lucent secretory granules were observed. Our results shows that the squamous-like cells can be differentiated into ciliated and secretory cells simultaneously by the floating method. (Korean J Otolaryngol 38:8, 1995)
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